Micellar Liquid Chromatographic Analysis for Simultaneous Determination of Atenolol and Hydrochlorothiazide in Tablet Dosage Form

Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate Micellar liquid chromatographic method was developed and validated for the simultaneous determination of atenolol and hydrochlorothiazide from bulk and and formulations. Method: The method uses C18 stationary phases and micellar mobile phases of 0.07M sodium dodecyl sulfate (SDS) pH 3 adjusted with phosphate buffer and 15% (v/v) 1propanol as organic modifier and ultraviolet detection at 225 nm are used for the determination. Results: Under these conditions, the studied atenolol and hydrochlorothiazide elute between 6.642 ± 0.10 and 2.467 ± 0.01 min at a 1.5 mL/min flow rate. The method showed excellent linearity in the range of 4–48 μg/mL and 1–12 μg/mL with the limit of detection (S/N = 3) 2 μg/mL and 0.1 μg/mL for atenolol and hydrochlorothiazide, respectively. The suggested method was successfully applied to the analysis of the studied atenolol and hydrochlorothiazide in tablet formulation with the respective average recoveries of 99.77 ± 0.20 and 99.55 ± 0.27%. Conclusion: The method developed can be used for the routine analysis of atenolol and hydrochlorothiazide from their combined dosage form.